Isolation, Partial Purification, and Characterization of Lectins from the Roots of <I>Vigna unguiculata</I> (L.) Walp.
Abstract
A lectin was isolated from the roots of 7-day old Vigna unguiculata (L.) Walp. seedlings using size-exclusion chromatography. The root lectin revealed two subunits in SDS-PAGE and migrated as a single protein in a pH gradient isoelectrofocusing gel electrophoresis as well as in native gel electrophoresis. The root lectin exhibited no specificity to any of the erythrocytes tested. In addition, the agglutinating activity of the root lectin was markedly increased by enzymes. The hemeagglutinating activity of the root lectin was inhibited by the sugars N-acetyl-galactosamine and methyl-a-D-glucopyranoside.
Published
2007-09-20
Issue
Section
Articles
Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that its publication has been approved by all co-authors, if any, as well as by the responsible authorities at the institute where the work has been carried out; that, if and when the manuscript is accepted for publication, the authors agree to the automatic transfer of the copyright to the publisher; that the manuscript will not be published elsewhere in any language without the consent of the copyright holders; that written permission of the copyright holder is obtained by the authors for material used from other copyrighted sources; and that any costs associated with obtaining this permission are the authors’ responsibility.
